Acetoin Fermentation by Citrate - Positive Lactococcus lactis subsp . lactis 3022 Grown Aerobically in the Presence of Hemin or Cu 2 +

Citr+ Lactococcus lactis subsp. lactis 3022 produced more biomass and converted most of the glucose substrate to diacetyl and acetoin when grown aerobically with hemin and Cu2". The activity of diacetyl synthase was greatly stimulated by the addition of hemin or Cu2+, and the activity of NAD-dependent diacetyl reductase was very high. Hemin did not affect the activities ofNADH oxidase and lactate dehydrogenase. These results indicated that the pyruvate formed via glycolysis would be rapidly converted to diacetyl and that the diacetyl would then be converted to acetoin by the NAD-dependent diacetyl reductase to reoxidize NADH when the cells were grown aerobically with hemin or Cu2+. On the other hand, the YGIU value for the hemincontaining culture was lower than for the culture without hemin, because acetate production was repressed when an excess of glucose was present. However, in the presence of lipoic acid, an essential cofactor of the dihydrolipoamide acetyltransferase part of the pyruvate dehydrogenase complex, hemin or Cu2+ enhanced acetate production and then repressed diacetyl and acetoin production. The activity of diacetyl synthase was lowered by the addition of lipoic acid. These results indicate that hemin or Cu2+ stimulates acetyl coenzyme A (acetyl-CoA) formation from pyruvate and that lipoic acid inhibits the condensation of acetyl-CoA with hydroxyethylthiamine PP;. In addition, it appears that acetyl-CoA not used for diacetyl synthesis is converted to acetate.